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The longevity of plant flowers is controlled by genetically programmed mechanisms. After a certain amount of time after flowering, the expression of genes associated with programmed cell death is induced and cellular components such as proteins and nucleic acids are degraded, causing the flower to wilt and die. In the horticultural field, flower longevity is an important trait that determines the commercial value of ornamental plants, and the development of technologies to prolong flower longevity is desired.

In previous research, a research team identified a transcription factor EPHEMERAL1 as a regulator of flower longevity in the Japanese morning glory, and it was thought that if a chemical could be found that suppressed the function of EPHEMERAL1, it would be possible to extend flower longevity.

In this study, the same researchers succeeded in constructing an assay system that can detect the binding of EPH1 to DNA using a wheat cell-free protein synthesis system and an AlphaScreen system, a molecular interaction analysis technology that PROS has independently developed. Using the AlphaScreen system, they succeeded in isolating Everlastin1 and Everlastin2, compounds that inhibit the binding of EPH1 to DNA.

The analysis using the AlphaScreen system revealed that EPH1 binds to DNA by dimerization and that Everlastin1 and Everlastin2 inhibit this EPH1 dimerization, thereby reducing the ability of EPH1 to bind to DNA. Floating cut morning glory flowers in water with dissolved Everlastin1 or Everlastin2 inhibited DNA and protein degradation and prolonged the longevity of the flowers by approximately twofold.

The paper is published in the journal Nature Plants.

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