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A recent study released by researchers at North Carolina State University offers new insights and guidelines for the accurate estimation of plant genome size using flow cytometry.

This latest research revisits long-held assumptions, identifies sources of variation, and establishes best practices and reference standards, with updated genome sizes, to enhance the precision and reliability of genome size estimation. It also critically examines the methodologies used and provides guidelines to address inconsistencies and improve accuracy. The research is published in the Journal of the American Society for Horticultural Science.

The first study focused on fluorochrome effects. A variety of fluorochromes are used in plant flow cytometry, but their effectiveness in staining the entire genome, the impact of chromatin on staining efficacy, and the influence of buffers and plant metabolites on dye fluorescence remain largely unknown.

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